Kuhnle G, Spencer J P, Chowrimootoo G, Schroeter H, Debnam E S, Srai S K, Rice-Evans C, Hahn U
Department of Biochemistry, University of Leipzig, Germany.
Biochem Biophys Res Commun. 2000 May 27;272(1):212-7. doi: 10.1006/bbrc.2000.2750.
We have studied the absorption and metabolism of resveratrol in the jejunum in an isolated rat small intestine model. Only small amounts of resveratrol were absorbed across the enterocytes of the jejunum and ileum unmetabolised. The major compound detected on the serosal side was the glucuronide conjugate of resveratrol (96.5% +/- 4.6 of the amount absorbed) indicating the susceptibility of resveratrol to glucuronidation during transfer across the rat jejunum. The presence of the glucuronide was confirmed using HPLC-PDA and nanoES-MS/MS techniques. These findings suggest that resveratrol is most likely to be in the form of a glucuronide conjugate after crossing the small intestine and entering the blood circulation. This will have important implications for the biological functions of resveratrol in vivo.
我们在离体大鼠小肠模型中研究了白藜芦醇在空肠中的吸收和代谢情况。仅有少量未代谢的白藜芦醇穿过空肠和回肠的肠细胞被吸收。在浆膜侧检测到的主要化合物是白藜芦醇的葡萄糖醛酸共轭物（占吸收量的96.5%±4.6%），这表明白藜芦醇在穿过大鼠空肠的过程中易于发生葡萄糖醛酸化。使用高效液相色谱-光电二极管阵列检测法（HPLC-PDA）和纳喷雾电喷雾串联质谱法（nanoES-MS/MS）证实了葡萄糖醛酸共轭物的存在。这些发现表明，白藜芦醇在穿过小肠并进入血液循环后很可能以葡萄糖醛酸共轭物的形式存在。这将对白藜芦醇在体内的生物学功能产生重要影响。
