Tweedell K S, Williams D C
J Cell Sci. 1976 Nov;22(2):385-95. doi: 10.1242/jcs.22.2.385.
A scanning electron-microscope study has established that there are major modifications in the surface membranes of malignant cells transformed from the pronephric kidney of the frog. Tumours were induced in vivo after exposure of embryos Lucké Herpes virus. A pronephric cell line from normal embryos and 3 tumour cell lines derived from experimentally induced adenocarcinomas of the pronephros were observed. The normal embryonic kidney cells have their surfaces covered with long fingerlike microvilli throughout the cell cycle. In contrast, the surface of the pronephric tumour cells are mainly covered by multiple, ruffled or parallel straight microridges and short, stubby microvilli. These comparisons were made on cells subject to similar culture conditions. The topographic changes in the tumour cells are considered significant since the characteristic microridges and stubby villi were consistently found in all 3 pronephric tumour lines.
一项扫描电子显微镜研究证实，从青蛙前肾转化而来的恶性细胞的表面膜存在重大改变。在将胚胎暴露于勒克疱疹病毒后，在体内诱导出肿瘤。观察了来自正常胚胎的前肾细胞系以及3个源自实验诱导的前肾癌的肿瘤细胞系。正常胚胎肾细胞在整个细胞周期中其表面都覆盖着长指状微绒毛。相比之下，前肾肿瘤细胞的表面主要覆盖着多个褶皱或平行的直微嵴以及短而粗的微绒毛。这些比较是在处于相似培养条件下的细胞上进行的。肿瘤细胞的形态变化被认为具有重要意义，因为在所有3个前肾肿瘤系中都始终发现了特征性的微嵴和粗短绒毛。
