Hu G f, Kim H j, Xu C j, Riordan J F
Center for Biochemical and Biophysical Sciences and Medicine, Department of Radiology, Harvard Medical School, 250 Longwood Avenue, Boston, Massachusetts 02115, USA.
Biochem Biophys Res Commun. 2000 Jul 5;273(2):551-6. doi: 10.1006/bbrc.2000.2978.
Exogenous acidic and basic fibroblast growth factors undergo rapid nuclear translocation in human umbilical vein endothelial cells. When nuclear translocation reaches saturation, more than 70% of the internalized growth factors are in the nuclear fraction. Lysosomal inhibitors, such as leupeptin and chloroquine, and microtubule inhibitors including colchicine and 2-methoxyl-beta-estradiol neither increase nor decrease nuclear translocation. The results suggest that nuclear translocation of fibroblast growth factors does not require cytosolic accumulation or lysosomal processing and that the transportation of exogenous growth factors across the cytoplasm is independent of microtubules.
外源性酸性和碱性成纤维细胞生长因子在人脐静脉内皮细胞中会迅速发生核转位。当核转位达到饱和时，超过70%内化的生长因子存在于核部分。溶酶体抑制剂，如亮抑酶肽和氯喹，以及微管抑制剂，包括秋水仙碱和2-甲氧基-β-雌二醇，既不增加也不减少核转位。结果表明，成纤维细胞生长因子的核转位不需要胞质积累或溶酶体加工，并且外源性生长因子穿过细胞质的运输与微管无关。
