Hoogstraten D, Qiao X, Sun Y, Hu A, Onodera S, Mindich L
Department of Microbiology, The Public Health Research Institute, 455 First Avenue, New York, New York 10016, USA.
Virology. 2000 Jun 20;272(1):218-24. doi: 10.1006/viro.2000.0374.
The three double-stranded RNA genomic segments of bacteriophage Phi8 were copied as cDNA, and their nucleotide sequences were determined. Although the organization of the genome is similar to that of Phi6, there is no similarity in either the nucleotide sequences or the amino acid sequences, with the exception of the motifs characteristic of viral RNA polymerases that are found in the presumptive polymerase sequence. Several features of the viral proteins differ markedly from those of Phi6. Although both phages are covered by a lipid-containing membrane, the protein compositions are very different. The most striking difference is that protein P8, which constitutes a shell around the procapsid in Phi6, is part of the membrane in Phi8. The host attachment protein consists of two peptides rather than one and the phage attaches directly to the lipopolysaccharide of the host rather than to a type IV pilus. The host range of Phi8 includes rough strains of Salmonella typhimurium and of pseudomonads
噬菌体Phi8的三个双链RNA基因组片段被转录为cDNA，并测定了它们的核苷酸序列。尽管该基因组的组织方式与Phi6相似，但除了在推测的聚合酶序列中发现的病毒RNA聚合酶的特征基序外，其核苷酸序列和氨基酸序列均无相似性。病毒蛋白的几个特征与Phi6明显不同。尽管两种噬菌体都被含脂质的膜覆盖，但蛋白质组成却大不相同。最显著的差异是，在Phi6中构成原衣壳周围外壳的蛋白质P8，在Phi8中是膜的一部分。宿主附着蛋白由两个肽段而非一个肽段组成，并且噬菌体直接附着于宿主的脂多糖而非IV型菌毛。Phi8的宿主范围包括鼠伤寒沙门氏菌的粗糙菌株和假单胞菌属
