Noriega N C, Hayes T B
Laboratory for Integrative Studies in Amphibian Biology, Group in Endocrinology, Deptartment of Integrative Biology, Museum of Vertebrate Zoology, University of California, Berkeley 94720-3104, USA.
Comp Biochem Physiol B Biochem Mol Biol. 2000 Jun;126(2):231-7. doi: 10.1016/s0305-0491(00)00201-7.
Organochlorine compounds such as o,p'DDT can mimic estrogen effects. We compared the effects of o,p'DDT and six other DDT congeners to the effects of estradiol by comparing in vivo color changes in the reed frog (Hyperolius argus). Premature female color pattern induction in H. argus is specific to estrogens and the current study suggests that this assay has potential for use in discriminating between xenobiotic estrogens and non-estrogens. Animals were treated at forelimb emergence and maintained in treated solution until final evaluation. Estradiol, o,p.DDT (0.1 microg/ml), o,p'DDE (1 microg/ml) and o,p'DDD (1 microg/ml) prematurely induced adult female coloration patterns in juvenile animals, whereas p,p'DDT, p, p'DDE and p,p'DDD did not.
有机氯化合物，如o,p’-滴滴涕，能够模拟雌激素的作用。我们通过比较芦苇蛙（Hyperolius argus）体内颜色变化，来对比o,p’-滴滴涕和其他六种滴滴涕同系物与雌二醇的作用。在芦苇蛙中，过早诱导雌性的颜色模式是雌激素所特有的，当前研究表明，该检测方法有潜力用于区分外源性雌激素和非雌激素。动物在出现前肢时接受处理，并一直置于处理液中直至最终评估。雌二醇、o,p-滴滴涕（0.1微克/毫升）、o,p’-滴滴滴（1微克/毫升）和o,p’-滴滴伊（1微克/毫升）可过早诱导幼年动物出现成年雌性的颜色模式，而p,p-滴滴涕、p,p’-滴滴滴和p,p’-滴滴伊则不能。
