Kashihara K, Akiyama K, Kodama M, Kohira I, Abe K
Department of Neurology, Okayama University Medical School, Japan.
Neurol Res. 2000 Jun;22(4):409-12. doi: 10.1080/01616412.2000.11758606.
We studied the temporal changes in expression of neuronal nitric oxide (NO) synthase (nNOS) mRNA in the hippocampus of rats treated with kainic acid by use of in situ hybridization technique. Intraperitoneal injection of 10 mg kg-1 kainic acid decreased expression of nNOS mRNAs in the dentate gyrus and CA3 region of the hippocampus at 3 h and 8 h and increased it in the dentate gyrus and CA1 at one week after treatment. Although our previous study indicated that administration of kainic acid increased NO generation in the rat hippocampus, present results suggest that the injection of kainic acid results in differential regulation of nNOS mRNA and NO formation in the rat hippocampus.
我们运用原位杂交技术研究了用 kainic 酸处理的大鼠海马中神经元型一氧化氮（NO）合酶（nNOS）mRNA 表达的时间变化。腹腔注射 10mg/kg 的 kainic 酸在处理后 3 小时和 8 小时降低了海马齿状回和 CA3 区 nNOS mRNA 的表达，并在处理后一周增加了齿状回和 CA1 区的表达。尽管我们之前的研究表明给予 kainic 酸会增加大鼠海马中的 NO 生成，但目前的结果表明注射 kainic 酸会导致大鼠海马中 nNOS mRNA 和 NO 形成的差异调节。
