[Kinetics and morphology of immune reactve cells in the spleen as observed uwing the immune adherence method. III. Spontaneous immunocytoadherence as influenced by total body irradiation].

The course of spontaneous immunocytoadherence as influenced by a total body 850 r irradiation was studied in the mouse spleen. Prior to the total body irradiation 0.87 RFC/100 cells occurring spontaneously, without any previous antigenic stimulus, were dislosed in the spleen. Twenty four hours after iraadiation 0.075 RFC/1000 cells were detected. This number dropped to 0.023 RFC/1000 cells at day 4 after irradiation. A qualitative morphological analysis showed the following values: 65,1% of the spontaneous RFC were small lymphocytes, 24.5% were medium-sized lymphocytes and 3% large lymphocytes; 1.1% were plasma cells and 5.4% macrophages. Twenty four hours after irradiation with a dose of 850 r the RFC contained 9.3% plasma cells, and 90.7% macrophages, the respective numbers on day 2 being 7.5% and 98.3%. At day 4, permitting to demonstrate the RFC in significant numbers there occurred macrophages only. The model experiment has shown that the formation of spontaneous rosettes represents a cirtually normal immune reaction.