Kimoto-Kinoshita S, Nishida S, Tomura T T
Department of Biochemistry & Oncology, Kinki University School of Medicine, Osaka-sayama, 589-8511, Osaka, Japan.
Neurosci Lett. 2000 Jul 14;288(2):103-6. doi: 10.1016/s0304-3940(00)01227-1.
We reported in our previous study that constitutive nitric oxide synthase (cNOS) activity significantly decreased and oxygen stress increased in the 31-week-old stroke-prone spontaneously hypertensive rat (SHRSP) cerebral cortex (CC). In the present study we examined the protein amount of two cNOS isoforms, the neuronal and the endothelial types, in SHRSP CC using Western blot analysis. Although no significant difference was observed in the amount of neuronal NOS (nNOS) protein, endothelial NOS (eNOS) protein prominently decreased in 31-week-old SHRSP CC compared to age-matched Wistar Kyoto rat and 15-week-old SHRSP. In rats at this age, we also observed a large quantity of albumin in the protein amount. However, the protein amount of heat shock protein 70, which is a molecular chaperon and a marker of injury, showed no significant changes. These results indicate that the alteration of eNOS but not of nNOS protein would be more closely associated with the development of stroke in SHRSP.
我们在之前的研究中报道，在31周龄的易中风自发性高血压大鼠（SHRSP）的大脑皮层（CC）中，组成型一氧化氮合酶（cNOS）活性显著降低，氧化应激增加。在本研究中，我们使用蛋白质印迹分析检测了SHRSP大脑皮层中两种cNOS亚型（神经元型和内皮型）的蛋白质含量。虽然在神经元型一氧化氮合酶（nNOS）蛋白含量上未观察到显著差异，但与年龄匹配的Wistar Kyoto大鼠和15周龄的SHRSP相比，31周龄SHRSP大脑皮层中的内皮型一氧化氮合酶（eNOS）蛋白显著减少。在这个年龄段的大鼠中，我们还观察到蛋白含量中有大量白蛋白。然而，作为分子伴侣和损伤标志物的热休克蛋白70的蛋白含量没有显著变化。这些结果表明，eNOS而非nNOS蛋白的改变可能与SHRSP中风的发生更密切相关。
