冷冻断裂放射自显影术。

Freeze-fracture-autoradiography.

作者信息

Rix E, Schiller A, Taugner R

出版信息

Histochemistry. 1976;50(2):91-101. doi: 10.1007/BF00495820.

Abstract

A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing. The advantages and limitations of the new method are discussed in terms of monolayer quality, contact, histochemography, resolution, freezing and recrystallisation artefacts.

摘要

本文描述了一种用于冷冻组织中可溶性物质的电子显微镜放射自显影的新方法。该方法的基本特征包括冷冻断裂、施加合适的单层膜，随后在低温下进行曝光，最后在显影处理后分离组织和复制单层膜三明治结构。从单层膜质量、接触情况、组织化学成像、分辨率、冷冻和重结晶伪像等方面讨论了该新方法的优缺点。