用于组织样本中可溶性物质定位的冷冻断裂放射自显影技术的改进
Improvement of freeze-fracture autoradiography for localization of soluble substances in tissue samples.
作者信息
Nobiling R, Bührle C P, Taugner R
出版信息
Histochemistry. 1985;82(1):45-9. doi: 10.1007/BF00502090.
PMID:3872289
Abstract
Freeze-fracture autoradiography is accepted as an adequate technique for localization studies of soluble substances at the electron microscopical level. The method, however, involves many critical preparation steps, among them a protective carbon coating of the developed nuclear emulsion adhering to the replica. We demonstrate here that this additional carbon coating may be omitted. This simplification leads to a significant improvement of the sample yield as compared with the previously described procedures.
摘要
冷冻断裂放射自显影术被认为是在电子显微镜水平上对可溶性物质进行定位研究的一种适当技术。然而，该方法涉及许多关键的制备步骤，其中包括在附着于复制品的显影核乳剂上进行保护性碳涂层处理。我们在此证明，这种额外的碳涂层可以省略。与先前描述的程序相比，这种简化导致样品产量显著提高。
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本文引用的文献
1
Freeze-fracture autoradiography of the red blood cell plasma membrane.红细胞质膜的冷冻断裂放射自显影术。
J Microsc. 1980 Apr;118(4):453-61. doi: 10.1111/j.1365-2818.1980.tb00295.x.
2
Monolayer freeze-fracture autoradiography: origins and directions.单层冷冻断裂放射自显影术：起源与方向
J Microsc. 1982 Apr;126(Pt 1):1-8. doi: 10.1111/j.1365-2818.1982.tb00353.x.
3
An improved method for freeze-fracture radioautography of tissues and cells, as applied to duodenal epithelium and thymic lymphocytes.一种用于组织和细胞冷冻断裂放射自显影的改进方法，应用于十二指肠上皮和胸腺淋巴细胞。
J Histochem Cytochem. 1984 Jan;32(1):17-29. doi: 10.1177/32.1.6690598.
4
Freeze-fracture-autoradiography.冷冻断裂放射自显影术。
Histochemistry. 1976;50(2):91-101. doi: 10.1007/BF00495820.
5
Freeze-fracture autoradiography: feasibility.冷冻断裂放射自显影术：可行性
J Cell Biol. 1976 Aug;70(2 pt 1):453-8. doi: 10.1083/jcb.70.2.453.
6
Freeze-fracture autoradiography: the in-vacuo coating technique.冷冻断裂放射自显影术：真空镀膜技术。
Histochemistry. 1978 Dec 28;59(1):9-16. doi: 10.1007/BF00506473.
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