Wauters M, Considine R, Löfgren A, Van Broeckhoven C, Van der Auwera J C, De Leeuw I, Van Gaal L
Department of Endocrinology, Metabolism, and Clinical Nutrition, University Hospital Antwerp, Belgium.
Metabolism. 2000 Jun;49(6):724-30. doi: 10.1053/meta.2000.6255.
Leptin levels have been shown previously to be associated with anthropometric parameters such as the body mass index (BMI), total body fat, and subcutaneous fat. Since apolipoprotein E (apoE) polymorphism is known to be a genetic marker affecting the relationship between certain anthropometric and metabolic parameters, we evaluated whether the leptin level and/or associations between the leptin level and body composition in non-insulin-dependent diabetic patients could be determined by apoE polymorphism. In 171 type 2 diabetic patients (105 male and 66 female), body composition (BMI, waist to hip ratio [WHR], fat mass, and visceral fat) was measured and fasting blood samples were obtained to determine the apoE genotype, leptin, glucose, and insulin levels, and the lipid profile. The mean leptin level for the whole group was 11.7 +/- 9.3 ng/mL, with a significant difference (P < .001) between men (7.1 +/- 4.9 ng/mL) and women (19.0 +/- 10.1 ng/mL). No difference was found for leptin levels or anthropometric variables between the 3 different apoE genotypes (E3/E3 homozygotes, E2 carriers, and E4 carriers). Only low-density lipoprotein (LDL) cholesterol was significantly different between the 3 apoE subgroups. The correlations of leptin with anthropometric variables, especially visceral fat, tended to be different between the 3 apoE groups, but this was not independent and no effect was found after controlling for the other parameters in the model. A multiple regression model containing gender, subcutaneous fat, fasting glucose, triglycerides, and high-density lipoprotein (HDL) cholesterol explained 81% of the variance in leptin levels. We conclude that apoE polymorphism has no effect on the leptin level or its associations with other anthropometric and metabolic parameters.
先前的研究表明，瘦素水平与人体测量参数相关，如体重指数（BMI）、全身脂肪和皮下脂肪。由于载脂蛋白E（apoE）多态性是影响某些人体测量和代谢参数之间关系的遗传标记，我们评估了非胰岛素依赖型糖尿病患者的瘦素水平和/或瘦素水平与身体组成之间的关联是否可由apoE多态性决定。在171例2型糖尿病患者（105例男性和66例女性）中，测量了身体组成（BMI、腰臀比[WHR]、脂肪量和内脏脂肪），并采集空腹血样以确定apoE基因型、瘦素、葡萄糖、胰岛素水平和血脂谱。整个组的平均瘦素水平为11.7±9.3 ng/mL，男性（7.1±4.9 ng/mL）和女性（19.0±10.1 ng/mL）之间存在显著差异（P <.001）。在3种不同的apoE基因型（E3/E3纯合子、E2携带者和E4携带者）之间，瘦素水平或人体测量变量没有差异。只有低密度脂蛋白（LDL）胆固醇在3个apoE亚组之间有显著差异。在3个apoE组中，瘦素与人体测量变量（尤其是内脏脂肪）的相关性往往不同，但这并非独立因素，在模型中控制其他参数后未发现影响。一个包含性别、皮下脂肪、空腹血糖、甘油三酯和高密度脂蛋白（HDL）胆固醇的多元回归模型解释了瘦素水平81%的方差。我们得出结论，apoE多态性对瘦素水平或其与其他人体测量和代谢参数的关联没有影响。
