在中国南方蜱虫中通过巢式PCR鉴定查菲埃立克体。
Identification of Ehrlichia chaffeensis by nested PCR in ticks from Southern China.
作者信息
Cao W C, Gao Y M, Zhang P H, Zhang X T, Dai Q H, Dumler J S, Fang L Q, Yang H
机构信息
Institute of Microbiology and Epidemiology, Beijing 100071, China.
出版信息
J Clin Microbiol. 2000 Jul;38(7):2778-80. doi: 10.1128/JCM.38.7.2778-2780.2000.
Abstract
A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55. 2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.
摘要
采用巢式聚合酶链反应（nested PCR）检测从中国南方采集的717只蜱虫，以确定是否存在查菲埃立克体（Ehrlichia chaffeensis）。29只成年龟形花蜱（Amblyomma testudinarium）中有16只（55.2%）检测呈阳性，240只成年和至少215只若虫（混合样本）的新血蜱（Haemaphysalis yeni）中有28只（11.7%）检测呈阳性。其他四种蜱虫检测为阴性。通过DNA测序对选定的阳性扩增子进行了确认。
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