Shiba H, Kimura N, Takayama S, Hinata K, Suzuki A, Isogai A
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
Biosci Biotechnol Biochem. 2000 May;64(5):1016-24. doi: 10.1271/bbb.64.1016.
Self-incompatible (SI) Brassica rapa (syn. B. campestris) was transformed with an antisense SLG gene by using SLG8 cDNA isolated from the B. campestris S8 homozygote. Two transformed lines were obtained and analyzed. Northern blot and Western blot analyses revealed that endogenous SLG and SRK were greatly reduced of the transcriptional and translational levels in the transformant. Pollination experiments confirmed that their SI phenotype had broken down. In addition, the progeny with the antisense SLG gene, resulting from self- or cross-pollination of the transgenic plant, also showed the self-compatible phenotype. The breakdown of SI in the tranformants was due to the change in property of the stigma and not of the pollen. These results provide strong evidence that SLG and/or SRK is implicated in the pollen-stigma recognition of SI and that they act only as stigmatic factors.
利用从白菜S8纯合子中分离得到的SLG8 cDNA，将自交不亲和（SI）的白菜（syn. B. campestris）用反义SLG基因进行转化。获得了两个转化株系并进行分析。Northern杂交和Western杂交分析表明，转化体中内源性SLG和SRK在转录和翻译水平上均大幅降低。授粉实验证实它们的SI表型已经打破。此外，转基因植株自花授粉或异花授粉产生的携带反义SLG基因的后代也表现出自交亲和表型。转化体中SI的打破是由于柱头特性的改变而非花粉特性的改变。这些结果提供了强有力的证据，表明SLG和/或SRK参与了SI的花粉-柱头识别，并且它们仅作为柱头因子起作用。
