Yu M, Ismail M M, Qureshi M A, Dearth R N, Barnes H J, Saif Y M
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691, USA.
Avian Dis. 2000 Apr-Jun;44(2):297-304.
Intestinal samples from turkey poults affected with poult enteritis and mortality syndrome (PEMS) were examined for viruses by immune electron microscopy and double-stranded RNA virus genome electropherotyping. Turkey coronavirus (TCV), avian rotaviruses, reovirus, and a yet undefined small round virus (SRV) were detected. The SRV and TCV were isolated and propagated in turkey embryos. Challenge of specific-pathogen-free turkey poults with SRV, TCV, or both resulted in mortality and clinical responses similar to those of natural PEMS. Our experiments indicate that SRV and TCV are possibly important agents in the etiology of PEMS and the combination of these infections might result in outbreaks with high mortality. The severity of clinical signs and mortality of PEMS are postulated to be partly related to the virus agents involved in individual outbreaks.
通过免疫电子显微镜和双链RNA病毒基因组电泳分析，对患有雏火鸡肠炎和死亡综合征（PEMS）的火鸡雏鸡的肠道样本进行了病毒检测。检测到火鸡冠状病毒（TCV）、禽轮状病毒、呼肠孤病毒和一种尚未明确的小圆病毒（SRV）。SRV和TCV在火鸡胚胎中分离并增殖。用SRV、TCV或两者对无特定病原体的火鸡雏鸡进行攻毒，导致的死亡率和临床反应与自然发生的PEMS相似。我们的实验表明，SRV和TCV可能是PEMS病因中的重要病原体，这些感染的组合可能导致高死亡率的疫情爆发。PEMS的临床症状严重程度和死亡率据推测部分与个别疫情中涉及的病毒病原体有关。
