Gawron A J, Lunte S M
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66047, USA.
Electrophoresis. 2000 Jun;21(10):2067-73. doi: 10.1002/1522-2683(20000601)21:10<2067::AID-ELPS2067>3.0.CO;2-5.
Capillary electrophoresis with UV detection was utilized to optimize copper complexation conditions for the analysis of neuropeptides. Complexation was confirmed by monitoring the response at a visible wavelength. Four complexation strategies were used to compare the UV response of native peptides and their respective copper complexes. All four strategies resulted in complete complexation, but on-capillary complexation provided significant advantages over precapillary and pre-/on-capillary. An increase in UV absorbance along with peak stacking resulted in a significantly greater response using the on-capillary technique. Also, on-capillary complexation does not require dilution of the sample. The effects of temperature and copper concentration were also investigated. The utility of this method for the separation of an enkephalin peptide mixture is presented.
采用紫外检测的毛细管电泳技术来优化用于分析神经肽的铜络合条件。通过监测可见波长处的响应来确认络合情况。使用了四种络合策略来比较天然肽及其各自铜络合物的紫外响应。所有四种策略均导致完全络合，但柱上络合比柱前络合以及柱前/柱上络合具有显著优势。柱上络合时紫外吸光度增加以及峰堆积导致使用该技术时响应显著增强。此外，柱上络合不需要稀释样品。还研究了温度和铜浓度的影响。展示了该方法用于分离脑啡肽肽混合物的实用性。
