Wójcik Lena, Szostek Bogdan, Maruszak Wioleta, Trojanowicz Marek
Department of Chemistry, Warsaw University, Pasteura 1, PL-02-093 Warsaw, Poland.
Electrophoresis. 2005 Mar;26(6):1080-8. doi: 10.1002/elps.200406184.
A capillary electrophoretic method with UV detection for separation and quantitation of perfluorocarboxylic acids (PFCAs) from C6-PFCA to C12-PFCA has been developed. The optimization of measurement conditions included the choice of the most appropriate type and concentration of buffer in the background electrolyte (BGE), as well as the type and the content of an organic modifier. The optimal separation of investigated PFCAs was achieved with 50 mM phosphate buffer and 40% isopropanol in the BGE using direct UV detection. The optimum wavelength for direct UV detection was optimized at 190 nm. For indirect detection, several chromophores were studied. Five mM 3,5-Dinitrobenzoic acid (3,5-DNBA) in 20 mM phosphate buffer BGE and indirect UV detection at 280 nm gave the optimal detection and separation performance for the investigated PFCAs. The possibility of on-line preconcentration of solutes by stacking has been examined for indirect detection. The detection limits (LODs) determined for direct UV detection ranged from 2 microg/mL for C6-PFCA to 33 microg/mL for C12-PFCA. The LODs obtained for indirect UV detection were comparable to those obtained for direct UV detection.
已开发出一种采用紫外检测的毛细管电泳方法，用于分离和定量从C6 - 全氟羧酸（PFCA）到C12 - PFCA的全氟羧酸。测量条件的优化包括在背景电解质（BGE）中选择最合适的缓冲液类型和浓度，以及有机改性剂的类型和含量。使用直接紫外检测，在BGE中采用50 mM磷酸盐缓冲液和40%异丙醇可实现所研究PFCA的最佳分离。直接紫外检测的最佳波长优化为190 nm。对于间接检测，研究了几种发色团。在20 mM磷酸盐缓冲液BGE中加入5 mM 3,5 - 二硝基苯甲酸（3,5 - DNBA）并在280 nm进行间接紫外检测，可为所研究的PFCA提供最佳检测和分离性能。已研究了通过堆积对溶质进行在线预富集用于间接检测的可能性。直接紫外检测确定的检测限（LOD）范围为C6 - PFCA为2 μg/mL至C12 - PFCA为33 μg/mL。间接紫外检测获得的LOD与直接紫外检测获得的LOD相当。
