Ziaber J, Pasnik J, Baj Z, Pokoca L, Chmielewski H, Tchórzewski H
Department of Neurology, Military Medical Academy of Lódz, Poland.
J Investig Allergol Clin Immunol. 2000 Mar-Apr;10(2):98-101.
Tumor necrosis factor-alpha (TNF-alpha) in multiple sclerosis (MS) is responsible for peripheral blood leukocyte priming. The aim of this study was to evaluate fluorescein isothiocyanate (FITC)-labelled TNF binding ability by peripheral blood lymphocytes and polymorphonuclear neutrophils (PMNs) of MS patients, measured using flow cytometry (FACScan). Three groups of MS patients (total 34) were examined. Higher serum levels of TNF soluble receptors sp55 and sp75 were found in the MS patients during MS acute exacerbation (n = 10) and in chronic progressive forms (n = 11) as compared to MS remission (n = 13) and other neurological diseases (n = 14). Peripheral blood lymphocytes and PMNs of patients with acute exacerbation of MS bound TNF-FITC more effectively (p <0.01) as compared with the chronic progressive forms of MS, MS remission and other neurological diseases. The obtained results suggest a greater enhancement of TNF activity during MS acute exacerbation.
肿瘤坏死因子-α（TNF-α）在多发性硬化症（MS）中负责外周血白细胞的致敏。本研究的目的是通过流式细胞术（FACScan）测量MS患者外周血淋巴细胞和多形核中性粒细胞（PMN）对异硫氰酸荧光素（FITC）标记的TNF的结合能力。对三组MS患者（共34例）进行了检查。与MS缓解期（n = 13）和其他神经系统疾病（n = 14）相比，在MS急性加重期（n = 10）和慢性进展型（n = 11）的MS患者中发现血清中TNF可溶性受体sp55和sp75水平较高。与MS慢性进展型、MS缓解期和其他神经系统疾病相比，MS急性加重期患者的外周血淋巴细胞和PMN与TNF-FITC的结合更有效（p <0.01）。所得结果表明在MS急性加重期TNF活性增强更为明显。
