Meyer-Siegler K
Department of Urology, Bay Pines VA Medical Center, FL 33744, USA.
Cytokine. 2000 Jul;12(7):914-21. doi: 10.1006/cyto.2000.0682.
Macrophage migration inhibitory factor (MIF) is a cytokine expressed by a number of different cell types and has been detected in prostatic glandular epithelial cells by immunohistochemistry. The goal of this study was to determine if in vitro cultured prostate cells produce this protein and some of the effects of MIF on these cells. Proliferation of normal prostate cells, the BPH-1 and DU-145 established cell lines in the presence of MIF were assessed. ELISA was used to screen conditioned medium for the production of MIF, matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2). Zymogram electrophoresis gels determined the activities of secreted MMP-2. The amount of MIF in the conditioned medium detected after 72 h of growth in normal, BPH-1 and DU-145 cells was 2.9, 5.2 and 10.2 ng/ml/10(6)cells respectively. Exogenous addition of MIF (25 ng/ml) to cells cultured in vitro stimulated proliferation of all the cell types tested. MIF addition to proliferating DU-145 cells resulted in a two-fold increase in the relative amount of active MMP-2 as determined by zymogram gel analysis of conditioned medium.
巨噬细胞移动抑制因子（MIF）是一种由多种不同细胞类型表达的细胞因子，免疫组织化学已在前列腺腺上皮细胞中检测到该因子。本研究的目的是确定体外培养的前列腺细胞是否产生这种蛋白质以及MIF对这些细胞的一些影响。评估了正常前列腺细胞、已建立的BPH-1和DU-145细胞系在MIF存在下的增殖情况。采用酶联免疫吸附测定（ELISA）筛选条件培养基中MIF、基质金属蛋白酶-2（MMP-2）和金属蛋白酶组织抑制剂-2（TIMP-2）的产生情况。酶谱电泳凝胶测定分泌型MMP-2的活性。正常细胞、BPH-1细胞和DU-145细胞生长72小时后，条件培养基中检测到的MIF量分别为2.9、5.2和10.2 ng/ml/10(6)个细胞。向体外培养的细胞中额外添加MIF（25 ng/ml）可刺激所有测试细胞类型的增殖。通过对条件培养基进行酶谱凝胶分析确定，向增殖的DU-145细胞中添加MIF导致活性MMP-2的相对量增加了两倍。
