Pang Z J, Chen Y, Zhou M
Research Laboratory of Free Radical Medicine, The First Military Medical University, Guangzhou, People's Republic of China.
Cytokine. 2000 Jul;12(7):944-50. doi: 10.1006/cyto.2000.0683.
We have previously found that L929 cell conditioned medium (L929-CM) could protect mouse peritoneal macrophages from oxidative injury. To uncover the mechanism further, we investigated the effect of L929-CM on the oxidative injury caused by tbOOH to RAW264.7 cell lines. The results showed that L929-CM could protect RAW264.7 cells from oxidative injury (presented by cell morphology and cell survival rate), and L929-CM could also improve total superoxide dismutase (SOD), selenium-dependent and non-selenium-dependent glutathione peroxidase (SeGPx and non-SeGPx) activities in RAW264.7 cells. RT-PCR analysis showed that, L929-CM could induce plasma glutathione peroxidase (PLGPx) mRNA expression, while there was no inducing effect of L929-CM on phospholipid hydroperoxide glutathione peroxidase (PHGPx) mRNA expression in RAW264.7 cells. 5 microg/ml actinomycin D, 30 microg/ml cycloheximide (de novo protein synthesis inhibitor) and 50 microg/ml acetovanilone (intracellular superoxide anion production inhibitor) had no effects in attenuating the induction of PLGPx expression by L929-CM.
我们之前发现，L929细胞条件培养基（L929-CM）可以保护小鼠腹腔巨噬细胞免受氧化损伤。为了进一步揭示其机制，我们研究了L929-CM对叔丁基过氧化氢（tbOOH）所致RAW264.7细胞系氧化损伤的影响。结果显示，L929-CM可以保护RAW264.7细胞免受氧化损伤（通过细胞形态和细胞存活率体现），并且L929-CM还可以提高RAW264.7细胞中总超氧化物歧化酶（SOD）、硒依赖性和非硒依赖性谷胱甘肽过氧化物酶（SeGPx和非SeGPx）的活性。逆转录聚合酶链反应（RT-PCR）分析表明，L929-CM可以诱导血浆谷胱甘肽过氧化物酶（PLGPx）mRNA表达，而L929-CM对RAW264.7细胞中磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPx）mRNA表达没有诱导作用。5微克/毫升放线菌素D、30微克/毫升环己酰亚胺（从头合成蛋白抑制剂）和50微克/毫升乙酰香草酮（细胞内超氧阴离子产生抑制剂）对减弱L929-CM诱导的PLGPx表达均无作用。
