在用曲古抑菌素a处理后，胰岛素样生长因子-II（IGF-II）和白细胞介素-2（IL-2）协同作用以改变组蛋白去乙酰化酶1（HDAC1）的表达。

IGF-II and IL-2 act synergistically to alter HDAC1 expression following treatments with trichostatin a.

作者信息

Gray S G, Svechnikova I, Hartmann W, O'Connor L, Aguilar-Santelises M, Ekström T J

机构信息

Laboratory for Molecular Development and Tumour Biology, Karolinska Institute CMM, L8 01, Stockholm, Sweden.

出版信息

Cytokine. 2000 Jul;12(7):1104-9. doi: 10.1006/cyto.2000.0680.

DOI:10.1006/cyto.2000.0680

PMID:10880258

Abstract

Histone deacetylases play key roles in the regulation of gene transcription. Studies have shown that expression of interleukins IL-2 and IL-8, and insulin-like growth factor 2 (IGF2) are affected by treatment with histone deacetylase inhibitors. We have previously shown that the gene for histone deacetylase 1 (HDAC1) is upregulated following treatment with TSA. The murine homologue of this gene has been reported to be inducible by IL-2. In this study, we have examined the effects IL-2, IGF-II and TSA have on HDAC1 expression in the human hepatocellular carcinoma derived cell line Hep3B. Our results indicate that in contrast to the mouse, HDAC1 is not inducible by IL-2. However, in TSA treated cells, IL-2 and IGF-II were found to act synergistically to reduce TSA induced HDAC1 mRNA levels almost to normal.

摘要

组蛋白去乙酰化酶在基因转录调控中起关键作用。研究表明，白细胞介素IL-2和IL-8以及胰岛素样生长因子2（IGF2）的表达受组蛋白去乙酰化酶抑制剂处理的影响。我们之前已经表明，用曲古抑菌素A（TSA）处理后，组蛋白去乙酰化酶1（HDAC1）基因会上调。据报道，该基因的小鼠同源物可被IL-2诱导。在本研究中，我们检测了IL-2、IGF-II和TSA对人肝癌衍生细胞系Hep3B中HDAC1表达的影响。我们的结果表明，与小鼠不同，HDAC1不能被IL-2诱导。然而，在经TSA处理的细胞中，发现IL-2和IGF-II具有协同作用，可将TSA诱导的HDAC1 mRNA水平降低至几乎正常。