Meléndez A, Greenwald I
Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Genetics. 2000 Jul;155(3):1127-37. doi: 10.1093/genetics/155.3.1127.
The SynMuv genes appear to be involved in providing a signal that inhibits vulval precursor cells from adopting vulval fates in Caenorhabditis elegans. One group of SynMuv genes, termed class B, includes genes encoding proteins related to the tumor suppressor Rb and RbAp48, a protein that binds Rb. Here, we provide genetic evidence that lin-13 behaves as a class B SynMuv gene. We show that null alleles of lin-13 are temperature sensitive and maternally rescued, resulting in phenotypes ranging in severity from L2 arrest (when both maternal and zygotic activities are removed at 25 degrees ), to sterile Multivulva (when only zygotic activity is removed at 25 degrees ), to sterile non-Multivulva (when both maternal and zygotic activities are removed at 15 degrees ), to wild-type/class B SynMuv (when only zygotic activity is removed at 15 degrees ). We also show that LIN-13 is a nuclear protein that contains multiple zinc fingers and a motif, LXCXE, that has been implicated in Rb binding. These results together suggest a role for LIN-13 in Rb-mediated repression of vulval fates.
SynMuv基因似乎参与提供一种信号，该信号抑制秀丽隐杆线虫中生殖前体细胞分化为生殖命运。一类SynMuv基因，称为B类，包括编码与肿瘤抑制因子Rb及RbAp48相关蛋白质的基因，RbAp48是一种与Rb结合的蛋白质。在此，我们提供遗传学证据表明lin-13作为B类SynMuv基因发挥作用。我们发现lin-13的无效等位基因对温度敏感且可由母体拯救，其表型严重程度各异，从L2期停滞（当在25摄氏度时母体和合子活性均被去除），到不育的多生殖孔（当在25摄氏度时仅合子活性被去除），到不育的非多生殖孔（当在15摄氏度时母体和合子活性均被去除），再到野生型/B类SynMuv（当在15摄氏度时仅合子活性被去除）。我们还表明LIN-13是一种核蛋白，它包含多个锌指结构和一个与Rb结合有关的LXCXE基序。这些结果共同表明LIN-13在Rb介导的生殖命运抑制中发挥作用。
