山羊抗胸腺细胞球蛋白的制备与检测

Preparation and testing of goat anti--thymocyte globulin.

作者信息

Mears D C, Sheil A G, Kelly G E, Beerenfels P C

出版信息

Aust J Exp Biol Med Sci. 1976 Oct;54(5):431-9. doi: 10.1038/icb.1976.43.

DOI:10.1038/icb.1976.43

Abstract

This study details methods of production, purification and testing of large volumes of uniformly potent, non-toxic, anti-human thymocyte globulin (ATG). Plasma from 70 goats immunised with human thymic lymphocytes was fractionated by ammonium sulphate precipitation to its IgG component. Contaminating antibodies were removed by absorption methods. After fractionation and absorption, ATG retained its ability to inhibit the formation of rosettes by human lymphocytes with sheep red blood cells. Antibodies to human glomerular basement membrane were not detected in any tested preparation. Tests performed on monkeys, mice and rabbits revealed low toxicity.

摘要

本研究详细介绍了大量均匀有效的无毒抗人胸腺细胞球蛋白（ATG）的生产、纯化和检测方法。用人类胸腺淋巴细胞免疫的70只山羊的血浆通过硫酸铵沉淀法分离出其IgG成分。通过吸收法去除污染抗体。分级分离和吸收后，ATG保留了抑制人淋巴细胞与绵羊红细胞形成玫瑰花结的能力。在任何测试制剂中均未检测到针对人肾小球基底膜的抗体。对猴子、小鼠和兔子进行的测试显示毒性较低。