Chen J J, Sun M, Fang J, Liu H, Chen C Q, Wang D B
Shanghai Research Centre of Bilotechnology, Chinese Academy of Sciences.
Sheng Wu Gong Cheng Xue Bao. 2000 Jan;16(1):36-41.
On SGI workstation, we constructed two anti-hTNF alpha McAbs by means of homologous protein-structure-prediction method. And then, on the basis of relative experimental results and the surface properties of hTNF alpha and two McAbs, we performed the docking of hTNF alpha into two anti-hTNF alpha McAbs. In order to confirm the models, we prepared two hTNF alpha mutants designed according to the binding models, analysed and predicted the possible changes in complexes resulted from hTNF alpha mutations. The experimental analysis results proved these complex models. This will make the base of our next antibody humanization and/or reshape work.
在硅图形工作站上，我们通过同源蛋白质结构预测方法构建了两种抗人肿瘤坏死因子α单克隆抗体。然后，根据相关实验结果以及人肿瘤坏死因子α和两种单克隆抗体的表面特性，我们将人肿瘤坏死因子α对接至两种抗人肿瘤坏死因子α单克隆抗体中。为了验证模型，我们根据结合模型制备了两个人肿瘤坏死因子α突变体，分析并预测了人肿瘤坏死因子α突变导致的复合物中可能发生的变化。实验分析结果证实了这些复合物模型。这将为我们下一步的抗体人源化和/或重塑工作奠定基础。
