[HIV-1 Gag p17-p24蛋白的表达与特性分析]
[Expression and characterization of HIV-1 Gag p17-p24 protein].
作者信息
Li T Y, Jin N Y, Wang H W, Guo Z R, Fang H H, An R G, Yin Z
机构信息
Changchun University of Agriculture and Animal Sciences.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2000 Jan;16(1):65-8.
The biological and immunological characteristics of HIV-1 core protein p17-24 expressed by recombinant vaccinia viruses were studied. The results of indirect immunofluorescence assay (I-IFA), Western blot and dot ELISA showed that the two recombinants could express the p24 gag and p17-24 gag fusion proteins in infected cell lines respectively. The electromicroscope observation revealed that the expressed proteins could also assemble into virus-like particles. The recombinant vaccinia viruses can also stimulate mice for the formation of anti HIV-1 Gag p24 antibody. When infected with the recombinant viruses, the chromosome DNA ladder caused by the apoptosis of the BHK cell was observed.
对重组痘苗病毒表达的HIV-1核心蛋白p17-24的生物学和免疫学特性进行了研究。间接免疫荧光试验（I-IFA）、蛋白质印迹法和斑点酶联免疫吸附测定结果表明，两种重组体分别能在感染的细胞系中表达p24 gag和p17-24 gag融合蛋白。电镜观察显示，表达的蛋白也能组装成病毒样颗粒。重组痘苗病毒还能刺激小鼠产生抗HIV-1 Gag p24抗体。当用重组病毒感染时，观察到BHK细胞凋亡引起的染色体DNA梯状条带。
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