Marounek M, Kopecný J, Bartos S
Vet Med (Praha). 1979 Apr;24(4):233-8.
The method of cultivating rumen fluid in buffer with nutrient admixture under CO2 atmosphere is described. The method serves for the comparison and description of the properties of NPN sources for ruminants. Its applicability is demonstrated on the example of urea. During incubation, all parameters of the medium remain within an admissible range. Considering the analyses of the incubation medium, i. e. determination of the pH value, ammonia, urea, volatile fatty acids, total protein, and redox potential, it is recommended to monitor the course of fermentation for six hours and to take samples in the intervals of 0, 1, 2, 4 and 6 hours.
本文描述了在二氧化碳气氛下，于缓冲液中添加营养混合物培养瘤胃液的方法。该方法用于比较和描述反刍动物非蛋白氮源的特性。以尿素为例证明了其适用性。在培养过程中，培养基的所有参数均保持在允许范围内。考虑到对培养基的分析，即pH值、氨、尿素、挥发性脂肪酸、总蛋白和氧化还原电位的测定，建议监测发酵过程6小时，并在0、1、2、4和6小时的间隔时间取样。
