中枢给予的食欲素/下丘脑泌素激活大鼠的下丘脑-垂体-肾上腺轴。

Centrally administered orexin/hypocretin activates HPA axis in rats.

作者信息

Kuru M, Ueta Y, Serino R, Nakazato M, Yamamoto Y, Shibuya I, Yamashita H

机构信息

Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.

出版信息

Neuroreport. 2000 Jun 26;11(9):1977-80. doi: 10.1097/00001756-200006260-00034.

DOI:10.1097/00001756-200006260-00034

PMID:10884055

Abstract

The effects of i.c.v. administration of orexin/hypocretin on plasma ACTH, corticosterone and c-fos mRNA in the paraventricular nucleus (PVN) of the rat were examined. Plasma ACTH levels were markedly increased at 30 min after i.c.v. administration of orexin-A. Plasma corticosterone levels were significantly increased in a dose-related manner 30 min after i.c.v. administration of orexin-A and orexin-B. In situ hybridization histochemistry revealed that the induction of the c-fos mRNA in the parvocellular division of the PVN was increased in a dose-related manner 30 min after i.c.v. administration of orexin-A and orexin-B. These results suggest that central orexin/hypocretin activates hypothalamo-pituitary-adrenal (HPA) axis and may be involved in stress-induced activation of the HPA axis.

摘要

研究了脑室内注射食欲素/下丘脑泌素对大鼠血浆促肾上腺皮质激素（ACTH）、皮质酮以及室旁核（PVN）中c-fos信使核糖核酸（mRNA）的影响。脑室内注射食欲素-A后30分钟，血浆ACTH水平显著升高。脑室内注射食欲素-A和食欲素-B后30分钟，血浆皮质酮水平以剂量相关方式显著升高。原位杂交组织化学显示，脑室内注射食欲素-A和食欲素-B后30分钟，PVN小细胞部c-fos mRNA的诱导以剂量相关方式增加。这些结果表明，中枢食欲素/下丘脑泌素激活下丘脑-垂体-肾上腺（HPA）轴，可能参与应激诱导的HPA轴激活。