Schreiner C, Bui Q, Breglia R, Burnett D, Koschier F, Podhasky P, White R, Hoffman G, Schroeder R
Petroleum Product Stewardship Council, Washington, DC 20036, USA.
J Toxicol Environ Health A. 2000 Jun 9;60(3):169-84. doi: 10.1080/009841000156475.
A distillate of light catalytic reformed naphtha (CAS number 64741-63-5, LCRN-D) administered by inhalation was tested for reproductive and developmental toxicity in Sprague-Dawley rats, following a modified OECD Guideline 421, Reproductive/Developmental Toxicity Screening protocol. LCRN-D was administered as a vapor, 6 h/d, 7 d/wk at target concentrations of 0, 750, 2500 or 7500 ppm to female rats for approximately 6 wk from 2 wk prior to mating, during mating through gestational d 19, and to males beginning 2 wk prior to mating for approximately 7 consecutive weeks. Dams and litters were sacrificed on postnatal d 4 and males were sacrificed within the week after the last litter was necropsied. Parental systemic effects observed at the 7500 ppm exposure level included slightly lower body weights for males throughout the study. Increased kidney to body weight and increased liver to body weight ratio in male rats exposed to 7500 ppm LCRN-D may be related to slightly lower final mean body weights. Body and organ weight data for female rats in all exposure groups were comparable to controls. No test-material-related microscopic changes were observed in the reproductive organs or nasal turbinate tissue of either sex. Reproductive performance was unaffected by exposure to LCRN-D. The mating and fertility indices were 100% in all groups. There were no significant exposure-related differences in implantation sites or live pups per litter, and no gross abnormalities were observed in pups from treated dams. Pups born from LCRN-D-exposed dams showed comparable body weights and weight gain to control pups. The viability index on postpartum d 4 was > or =97%. Under conditions of this study, the no-observed-adverse-effect level (NOAEL) for exposure to light catalytic reformed naphtha distillate for parental effects was 2500 ppm and the NOAEL for reproductive and developmental toxicity was 7500 ppm.
按照经修订的经济合作与发展组织（OECD）准则421《生殖/发育毒性筛选方案》，对吸入给予的轻质催化重整石脑油馏出物（CAS编号64741-63-5，LCRN-D）进行了Sprague-Dawley大鼠生殖和发育毒性试验。从交配前2周开始，以蒸气形式给予LCRN-D，每天6小时，每周7天，目标浓度分别为0、750、2500或7500 ppm，雌性大鼠给药约6周，从交配前2周直至妊娠第19天，雄性大鼠从交配前2周开始给药约连续7周。产后第4天处死母鼠和幼崽，最后一窝幼崽尸检后的一周内处死雄性大鼠。在7500 ppm暴露水平观察到的亲代全身效应包括整个研究期间雄性大鼠体重略低。暴露于7500 ppm LCRN-D的雄性大鼠肾重与体重之比增加以及肝重与体重之比增加可能与最终平均体重略低有关。所有暴露组雌性大鼠的体重和器官重量数据与对照组相当。在任何性别的生殖器官或鼻甲组织中均未观察到与受试物相关的微观变化。生殖性能不受LCRN-D暴露的影响。所有组的交配和生育指数均为100%。着床部位或每窝活幼崽数无显著的与暴露相关差异，且未观察到受试母鼠所生幼崽有明显异常。LCRN-D暴露母鼠所生幼崽的体重和体重增加与对照幼崽相当。产后第4天的存活指数≥97%。在本研究条件下，轻质催化重整石脑油馏出物暴露的亲代效应未观察到有害作用水平（NOAEL）为2500 ppm，生殖和发育毒性的NOAEL为7500 ppm。
