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1
The asymmetric distribution of enzymic activity between the six subunits of bovine liver glutamate dehydrogenase. Use of D- and L-glutamyl alpha-chloromethyl ketones (4-amino-6-chloro-5-oxohexanoic acid.牛肝谷氨酸脱氢酶六个亚基间酶活性的不对称分布。D-和L-谷氨酰α-氯甲基酮（4-氨基-6-氯-5-氧代己酸）的应用。
Biochem J. 1976 Sep 1;157(3):675-86. doi: 10.1042/bj1570675.
2
[Identification of a glutamate dehydrogenase amino acid residue modified by 2,2,6,6-tetramethyl-4-oxopiperidine-1-oxyl. Study of the type of inactivation of a catalytically active enzyme oligomer in modification].[鉴定被2,2,6,6-四甲基-4-氧代哌啶-1-氧基修饰的谷氨酸脱氢酶氨基酸残基。修饰中催化活性酶寡聚体失活类型的研究]
Bioorg Khim. 1984 Sep;10(9):1171-6.
3
Affinity labeling of the catalytic subunit of cyclic AMP-dependent protein kinase by N alpha-tosyl-L-lysine chloromethyl ketone.用Nα-对甲苯磺酰-L-赖氨酸氯甲基酮对环磷酸腺苷依赖性蛋白激酶催化亚基进行亲和标记。
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3073-7. doi: 10.1073/pnas.76.7.3073.
4
Bovine liver glutamate dehydrogenase: tentative amino acid sequence; identification of a reactive lysine; nitration of a specific tyrosine and loss of allosteric inhibition by guanosine triphosphate.牛肝谷氨酸脱氢酶：暂定氨基酸序列；活性赖氨酸的鉴定；特定酪氨酸的硝化以及三磷酸鸟苷变构抑制作用的丧失
Proc Natl Acad Sci U S A. 1970 Oct;67(2):724-30. doi: 10.1073/pnas.67.2.724.
5
The site at which 4-iodoacetamidosalicylate reacts with glutamate dehydrogenases.4-碘乙酰氨基水杨酸与谷氨酸脱氢酶反应的位点。
Biochem J. 1973 May;133(1):165-71. doi: 10.1042/bj1330165.
6
Glutamate dehydrogenase: amino-acid sequence of the bovine enzyme and comparison with that from chicken liver.谷氨酸脱氢酶：牛源酶的氨基酸序列及其与鸡肝谷氨酸脱氢酶的比较
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1380-3. doi: 10.1073/pnas.69.6.1380.
7
Evidence for an active-center cysteine in the SH-proteinase alpha-clostripain through use of N-tosyl-L-lysine chloromethyl ketone.通过使用N-对甲苯磺酰-L-赖氨酸氯甲基酮证明SH蛋白酶α-梭菌蛋白酶中存在活性中心半胱氨酸。
FEBS Lett. 1984 Jul 23;173(1):58-62. doi: 10.1016/0014-5793(84)81017-0.
8
Ox liver glutamate dehydrogenase. The use of chemical modification to study the relationship between catalytic sites for different amino acid substrates and the question of kinetic non-equivalence of the subunits.牛肝谷氨酸脱氢酶。利用化学修饰研究不同氨基酸底物催化位点之间的关系以及亚基动力学不等价性的问题。
Biochem J. 1984 Sep 15;222(3):621-6. doi: 10.1042/bj2220621.
9
Bovine liver glutamate dehydrogenase. Equilibria and kinetics of imine formation by lysine-97 with pyridoxal 5'-phosphate.牛肝谷氨酸脱氢酶。赖氨酸-97与磷酸吡哆醛形成亚胺的平衡与动力学。
Biochemistry. 1971 Nov 23;10(24):4544-52. doi: 10.1021/bi00800a031.
10
Bovine liver glutamate dehydrogenase. Equilibria and kinetics of inactivation by pyridoxal.牛肝谷氨酸脱氢酶。吡哆醛失活的平衡与动力学。
Biochemistry. 1971 Nov 23;10(24):4538-44. doi: 10.1021/bi00800a030.

引用本文的文献

1
The structure and allosteric regulation of glutamate dehydrogenase.谷氨酸脱氢酶的结构和别构调节。
Neurochem Int. 2011 Sep;59(4):445-55. doi: 10.1016/j.neuint.2010.10.017. Epub 2010 Nov 9.
2
Ox liver glutamate dehydrogenase. The use of chemical modification to study the relationship between catalytic sites for different amino acid substrates and the question of kinetic non-equivalence of the subunits.牛肝谷氨酸脱氢酶。利用化学修饰研究不同氨基酸底物催化位点之间的关系以及亚基动力学不等价性的问题。
Biochem J. 1984 Sep 15;222(3):621-6. doi: 10.1042/bj2220621.
3
Specific labelling of a constituent polypeptide of bovine heart mitochondrial reduced nicotinamide-adenine dinucleotide-ubiquinone reductase by the inhibitor diphenyleneiodonium.抑制剂二苯撑碘鎓对牛心线粒体还原型烟酰胺腺嘌呤二核苷酸 - 泛醌还原酶的一种组成多肽的特异性标记
Biochem J. 1977 Jun 1;163(3):605-15. doi: 10.1042/bj1630605.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The oxidation of ribonuclease with performic acid.用过甲酸氧化核糖核酸酶。
J Biol Chem. 1956 Apr;219(2):611-21.
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The crystallization and characterization of L-glutamic acid dehydrogenase.L-谷氨酸脱氢酶的结晶与表征
J Biol Chem. 1952 May;197(1):67-79.
4
The subunits of bovine liver glutamate dehydrogenase: demonstration of a single peptide chain.牛肝谷氨酸脱氢酶的亚基：单条肽链的证明
J Mol Biol. 1966 Jun;18(1):77-89. doi: 10.1016/s0022-2836(66)80078-5.
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The reaction of glutamate dehydrogenase with 4-iodoacetamido salicylic acid.谷氨酸脱氢酶与4-碘乙酰氨基水杨酸的反应。
Eur J Biochem. 1970 Sep;15(3):555-61. doi: 10.1111/j.1432-1033.1970.tb01040.x.
6
Bovine liver flutamate dehydrogenase. Sequence of a hexadecapeptide containing a lysyl residue reactive with pyridoxal 5'-phosphate.牛肝谷氨酸脱氢酶。含一个与磷酸吡哆醛反应的赖氨酰残基的十六肽序列。
J Biol Chem. 1970 May 25;245(10):2622-6.
7
The preparation of chloromethylketone analogues of amino acids: inhibition of leucine aminopeptidase.氨基酸氯甲基酮类似物的制备：亮氨酸氨肽酶的抑制作用
Arch Biochem Biophys. 1972 Feb;148(2):447-51. doi: 10.1016/0003-9861(72)90163-4.
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Strategy and tactics in protein chemistry.蛋白质化学中的策略与战术。
Biochem J. 1970 Oct;119(5):805-22. doi: 10.1042/bj1190805f.
9
Selective chemical modification of proteins.蛋白质的选择性化学修饰。
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10
Investigation of the quaternary structure of beef liver glutamate dehydrogenase with bifunctional reagents.
Biochem Biophys Res Commun. 1974 Apr 23;57(4):1080-8. doi: 10.1016/0006-291x(74)90807-9.

牛肝谷氨酸脱氢酶六个亚基间酶活性的不对称分布。D-和L-谷氨酰α-氯甲基酮（4-氨基-6-氯-5-氧代己酸）的应用。

The asymmetric distribution of enzymic activity between the six subunits of bovine liver glutamate dehydrogenase. Use of D- and L-glutamyl alpha-chloromethyl ketones (4-amino-6-chloro-5-oxohexanoic acid.

作者信息

Rasool C G, Nicolaidis S, Akhtar M

出版信息

Biochem J. 1976 Sep 1;157(3):675-86. doi: 10.1042/bj1570675.

DOI:10.1042/bj1570675
PMID:10889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1163910/
Abstract

A method for the preparation of D- and L-glutamyl alpha-chloromethyl ketones (4-amino-6-chloro-5-oxohexanoic acid) is described. These chloromethyl ketones irreversibly inactivated bovine glutamate dehydrogenase, whereas several other related compounds had no adverse effect on the activity of the enzyme. The inactivation process was shown to be due to the modification of lysine-126. The time-courses for the inactivation and the incorporation of radioactivity from tritiated L-glutamyl alpha-chloromethyl ketone into the glutamate dehydrogenase were biphasic. The results were interpreted to suggest the involvement of 'negative co-operative' interactions in the reactivity of lysine-126. From the cumulative evidence it is argued that the first subunit of the enzyme, which takes part in catalysis, makes the largest, and the last the smallest, contribution to the overall catalysis. It is emphasized that three of the six subunits of the enzyme may possess as much as 80% of the total activity of bovine glutamate dehydrogenase.

摘要

描述了一种制备D-和L-谷氨酰α-氯甲基酮（4-氨基-6-氯-5-氧代己酸）的方法。这些氯甲基酮可使牛谷氨酸脱氢酶不可逆地失活，而其他几种相关化合物对该酶的活性没有不利影响。失活过程表明是由于赖氨酸-126的修饰所致。氚标记的L-谷氨酰α-氯甲基酮使谷氨酸脱氢酶失活以及放射性掺入的时间进程是双相的。结果表明赖氨酸-126的反应性涉及“负协同”相互作用。综合证据表明，参与催化的酶的第一个亚基对总体催化的贡献最大，而最后一个亚基的贡献最小。需要强调的是，该酶的六个亚基中的三个可能具有牛谷氨酸脱氢酶总活性的80%之多。