4-碘乙酰氨基水杨酸与谷氨酸脱氢酶反应的位点。
The site at which 4-iodoacetamidosalicylate reacts with glutamate dehydrogenases.
作者信息
Holbrook J J, Roberts P A, Wallis R B
出版信息
Biochem J. 1973 May;133(1):165-71. doi: 10.1042/bj1330165.
Abstract
- Bovine, porcine and chicken liver glutamate dehydrogenases were irreversibly inhibited by a tenfold excess of radioactive 4-iodoacetamidosalicylic acid at pH7.5. 2. Inhibition was accompanied by the covalent incorporation of 1.1 mol of labelled inhibitor/mol of polypeptide chain. Acid hydrolysis yielded N(epsilon)-carboxymethyl-lysine as the sole labelled amino acid. No labelled S-carboxymethylcysteine was recovered from the bovine or porcine enzymes. 3. The labelled bovine enzyme was hydrolysed with trypsin. The radioactivity was found at lysine-126 in a peptide comprising residues 119-130 of the sequence. 4. The amino acid compositions of the tryptic peptides containing labelled lysine from the porcine and chicken enzymes were similar to that of the bovine peptide.
摘要
- 在pH7.5条件下，过量10倍的放射性4-碘乙酰氨基水杨酸可使牛、猪和鸡肝脏的谷氨酸脱氢酶受到不可逆抑制。2. 抑制作用伴随着每摩尔多肽链共价结合1.1摩尔标记抑制剂。酸水解产生N（ε）-羧甲基赖氨酸作为唯一的标记氨基酸。从牛或猪的酶中未回收标记的S-羧甲基半胱氨酸。3. 用胰蛋白酶水解标记的牛酶。在包含序列119-130位残基的肽段中的赖氨酸-126处发现放射性。4. 来自猪和鸡酶的含标记赖氨酸的胰蛋白酶肽段的氨基酸组成与牛肽段相似。
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