Pereira C A, Monezi T A, Mehnert D U, D'Angelo M, Durigon E L
Institute of Biomedical Sciences, University of São Paulo, 1374, Av. Prof. Lineu Prestes, CEP 05508-900, São Paulo, Brazil.
Vet Microbiol. 2000 Jul 31;75(2):127-33. doi: 10.1016/s0378-1135(00)00214-5.
Canine parvovirus (CPV) was first isolated in 1978 in the USA. Analysis of CPV isolates by monoclonal antibodies and restriction enzymes have shown that after the first emergence of CPV (CPV-2) it evolved to give rise to new antigenic types, which were designated CPV type 2a and type 2b. These new types have replaced the original CPV type 2, although the proportions of each of the new antigenic types vary in different countries. In Brazil, CPV-like infections were first observed in 1979, however, there has been no information concerning the antigenic types of CPV prevailing in South America. In this study, we designed a PCR assay to type canine parvovirus strains in fecal samples collected from symptomatic dogs during 1980 through 1986 and 1990 through 1995. Our data showed that the CPV epizootic in Brazil followed the same pattern observed in the USA of emergence of CPV-2 followed by replacement by the variants CPV-2a and 2b. The predominant strain found during 1980 was CPV-2a, which was substantially replaced by CPV-2b from 1990 to 1995.
犬细小病毒（CPV）于1978年在美国首次分离出来。通过单克隆抗体和限制性内切酶对CPV分离株进行分析表明，在CPV首次出现（CPV-2）后，它进化产生了新的抗原类型，分别被命名为CPV 2a型和2b型。这些新类型已经取代了原来的CPV 2型，尽管每种新抗原类型在不同国家的比例有所不同。在巴西，1979年首次观察到类似CPV的感染，然而，关于南美洲流行的CPV抗原类型尚无相关信息。在本研究中，我们设计了一种PCR检测方法，用于对1980年至1986年以及1990年至1995年期间从出现症状的犬只收集的粪便样本中的犬细小病毒株进行分型。我们的数据表明，巴西的CPV流行遵循了在美国观察到的相同模式，即CPV-2出现后被CPV-2a和2b变体所取代。1980年发现的主要毒株是CPV-2a，从1990年到1995年，它基本上被CPV-2b所取代。
