Lord G A, Cai H, Luo J L, Lim C K
MRC Toxicology Unit, University of Leicester, UK.
Analyst. 2000 Apr;125(4):605-8. doi: 10.1039/a908996i.
The photodynamic therapeutic agent temoporfin, 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (m-THPC) conjugated with poly(ethylene glycol) 2000 (PEG), has been analysed by high performance liquid chromatography (HPLC), linked to electrospray ionisation mass spectrometry (ESI-MS). Sufficient separation of m-THPC-PEG 2000 oligomers was achieved, enabling determination of molecular mass. The use of ESI-MS alone could not achieve this, because of too great a complexity in the mass spectrum, resulting from the presence of four PEG 2000 side chains with a wide molecular mass distribution. The technique is applicable to similar PEG conjugated compounds.
已通过与电喷雾电离质谱（ESI-MS）联用的高效液相色谱（HPLC）对光动力治疗剂替莫泊芬（5,10,15,20-四（间羟基苯基）二氢卟酚，m-THPC）与聚乙二醇2000（PEG）共轭物进行了分析。实现了m-THPC-PEG 2000低聚物的充分分离，从而能够测定分子量。仅使用ESI-MS无法做到这一点，因为质谱过于复杂，这是由于存在四个分子量分布广泛的PEG 2000侧链所致。该技术适用于类似的PEG共轭化合物。
