Naryshkin N, Revyakin A, Kim Y, Mekler V, Ebright R H
Howard Hughes Medical Institute, Department of Chemistry, Rutgers University, Piscataway, New Jersey 08854, USA.
Cell. 2000 Jun 9;101(6):601-11. doi: 10.1016/s0092-8674(00)80872-7.
We have used systematic site-specific protein-DNA photocrosslinking to define interactions between bacterial RNA polymerase (RNAP) and promoter DNA in the catalytically competent RNAP-promoter open complex (RPo). We have mapped more than 100 distinct crosslinks between individual segments of RNAP subunits and individual phosphates of promoter DNA. The results provide a comprehensive description of protein-DNA interactions in RPo, permit construction of a detailed model for the structure of RPo, and permit analysis of effects of a transcriptional activator on the structure of RPo.
我们利用系统性的位点特异性蛋白质-DNA光交联技术来确定细菌RNA聚合酶（RNAP）与具有催化活性的RNAP-启动子开放复合物（RPo）中的启动子DNA之间的相互作用。我们已经绘制出RNAP亚基的各个片段与启动子DNA的各个磷酸基团之间100多个不同的交联点。这些结果全面描述了RPo中蛋白质-DNA的相互作用，有助于构建RPo结构的详细模型，并有助于分析转录激活剂对RPo结构的影响。
