Ishikura H, Kondo K, Miyoshi T, Kinoshita H, Hirose T, Monden Y
Second Department of Surgery, School of Medicine, Tokushima University, Tokushima City, Japan.
Ann Thorac Surg. 2000 Jun;69(6):1691-5. doi: 10.1016/s0003-4975(00)01144-9.
We established a new, patientlike orthotopic model of lung cancer metastasis with human non-small cell lung cancer cell lines. In this report, we describe the progressive stages of development of lymphogenous mediastinal metastasis in the Ma44-3 cell line from day 3 to day 15 after implantation in severe combined immunodeficiency mice and the process of lymphogenous metastasis.
All mice killed after day 12 had perivascular and peribronchial tumor growth. Micrometastasis to the mediastinum was first observed on day 5. On days 5 through 9, 10 of 13 mice had metastasis to the mediastinum, and all mice had one by day 12. When perivascular and peribronchial tumor growth was present by day 5, metastasis to the mediastinum developed in all mice.
This study demonstrates the lymphogenous spread of human lung cancer in severe combined immunodeficiency mouse using an orthotopic implantation model. Our model was thought to be an artificial lymphogenous metastasis model, owing to forced tumor inoculation into lymphatic vessels.
我们用人非小细胞肺癌细胞系建立了一种新的、类似患者的肺癌转移原位模型。在本报告中，我们描述了重度联合免疫缺陷小鼠植入Ma44 - 3细胞系后第3天至第15天纵隔淋巴源性转移的进展阶段以及淋巴转移过程。
第12天后处死的所有小鼠均有血管周围和支气管周围肿瘤生长。第5天首次观察到纵隔微转移。在第5天至第9天，13只小鼠中有10只发生纵隔转移，到第12天所有小鼠均有纵隔转移。当第5天出现血管周围和支气管周围肿瘤生长时，所有小鼠均发生纵隔转移。
本研究利用原位植入模型证明了人肺癌在重度联合免疫缺陷小鼠中的淋巴转移。由于将肿瘤强行接种到淋巴管中，我们的模型被认为是一种人工淋巴转移模型。
