Aihara H, Takasaki T, Toyosaki-Maeda T, Suzuki R, Okuno Y, Kurane I
Department of Microbiology, Kinki University School of Medicine, Osaka-sayama, Japan.
Viral Immunol. 2000;13(2):179-86. doi: 10.1089/vim.2000.13.179.
Mouse brain-derived inactivated Japanese encephalitis (JE) vaccine is the only currently internationally accepted vaccine against JE virus. We analyzed cellular and humoral immune responses to the JE vaccine in healthy adults in order to understand the protective immunity induced by this vaccine. Immunization with the JE vaccine induced T-cell activation in vivo, demonstrated by increase in the plasma levels of interleukin (IL)-2 and soluble CD8. JE virus-specific antibodies determined in radioimmunoprecipitation (RIP), hemagglutination inhibition (HI), and neutralization assays were also induced by immunization with the JE vaccine. JE virus-specific memory T cells were detected 60 days after immunization. These results suggest that protective immunity induced by the inactivated JE vaccine includes JE virus-specific T cells as well as antibodies with multiple biological activities.
鼠脑源性灭活日本脑炎（JE）疫苗是目前唯一国际认可的抗JE病毒疫苗。我们分析了健康成年人对JE疫苗的细胞免疫和体液免疫反应，以了解该疫苗诱导的保护性免疫。用JE疫苗免疫可在体内诱导T细胞活化，这通过血浆中白细胞介素（IL）-2和可溶性CD8水平的升高得以证明。通过放射免疫沉淀（RIP）、血凝抑制（HI）和中和试验测定的JE病毒特异性抗体也可由JE疫苗免疫诱导产生。免疫60天后可检测到JE病毒特异性记忆T细胞。这些结果表明，灭活JE疫苗诱导的保护性免疫包括JE病毒特异性T细胞以及具有多种生物学活性的抗体。
