人谷胱甘肽 - S - 转移酶：在乳酸乳球菌中的克隆与表达
Human glutathione-S-transferase: cloning and expression in Lactococcus lactis.
作者信息
Xiang H, Wei W, Zhang Y, Tan H
机构信息
Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China.
出版信息
Biomol Eng. 2000 Jun;16(6):207-9. doi: 10.1016/s1389-0344(99)00055-6.
Abstract
The glutathione-S-transferase A1 cDNA was amplified from the total RNAs of human liver by RT-PCR, and inserted into plasmid pMG36e. The hGSTA1 was expressed in Lactococcus lactis MG1363 and verified by SDS-PAGE and Western blot, purified by affinity chromatography and showed enzymatic activity.
摘要
通过逆转录聚合酶链反应（RT-PCR）从人肝脏的总RNA中扩增出谷胱甘肽-S-转移酶A1（glutathione-S-transferase A1）的互补DNA（cDNA），并将其插入质粒pMG36e中。人谷胱甘肽-S-转移酶A1（hGSTA1）在乳酸乳球菌MG1363中表达，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质免疫印迹法（Western blot）进行验证，通过亲和层析法进行纯化并显示出酶活性。
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