Nevils M A, Figueroa J V, Turk J R, Canto G J, Le V, Ellersieck M R, Carson C A
Department of Veterinary Patholobiology, College of Veterinary Medicine, University of Missouri, Columbia 65211, USA.
Parasitol Res. 2000 Jun;86(6):437-43. doi: 10.1007/s004360050691.
Clones of a Babesia bovis isolate known to cause particularly severe cerebral babesiosis were tested for virulence phenotype by inoculation of cattle. Clones were selected for phenotyping by two criteria - rate of growth in culture and hybridization of a virulence-related probe to Southern blots. Largely on the basis of associated mortality, B. bovis clones were judged to vary in their pathogenic potential.
通过给牛接种，对一种已知会引发特别严重的脑型巴贝斯虫病的牛巴贝斯虫分离株的克隆进行了毒力表型测试。根据两个标准选择克隆进行表型分析——培养中的生长速率以及与毒力相关探针与Southern印迹的杂交情况。主要基于相关的死亡率，判断牛巴贝斯虫克隆在致病潜力方面存在差异。
