Unusual organization of the 5S ribosomal spacer in Dirofilaria repens: absence of a canonical spliced leader 1 sequence.
作者信息
Favia G, Bazzocchi C, Cancrini G, Genchi C, Bandi C
机构信息
Istituto di Patologia Generale Veterinaria dell'Università degli Studi di Milano, Milan, Italy.
出版信息
Parasitol Res. 2000 Jun;86(6):497-9. doi: 10.1007/s004360050700.
PMID:10894477
Abstract
Comparing the 5S ribosomal RNA spacer of Dirofilaria repens and D. immitis, we found that in D. repens PCR generates two amplification products. In both products, the putative spliced leader 1 sequences (SL1) show some nucleotide substitutions compared to the canonical SL1 sequence of nematodes. SL I is a short sequence transferred to the 5' end of most messenger RNAs through a trans-splicing reaction. All the other filariae studied thus far amplify a single fragment showing a canonical SL1 sequence. D. repens appears thus peculiar.
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