Illouz S, Boubli L, Lavaut M N, Allasia C, Charpin C
Pathology Department, School of Medicine, University of Aix-Marseille II, Marseille, France.
Gynecol Obstet Invest. 2000;50(1):43-9. doi: 10.1159/000010279.
The objective of this study was to evaluate the endometrial response to sexual steroids in organ culture using two means: prostaglandin (PG) F(2alpha) output in medium culture and steroid receptor immunoexpression in tissue. Human endometrium samples were classified in homogeneous and heterogeneous proliferative or secretory subsets. In proliferative endometrium explant culture, progesterone (10(-7) M) induced a significant decrease in PGF(2alpha) output, but this was not the case in secretory endometrium, whereas no significant effect of estradiol (10(-8) M) was observed. Before culture, homogeneous and heterogeneous proliferative endometrium presented the same pattern of estradiol receptor (ER) and progesterone receptor (PR) expression evaluated by quantitative immunocytochemistry. After culture, immunoreactive ER and PR were detected on the explant. PR immunoexpression rates after culture were lower than before culture in glands on homogeneous proliferative and in stroma on heterogeneous proliferative endometrium explants without in vitro steroid addition. In secretory endometrium, no significant difference was observed between ER or PR immunoexpression rates before culture and after culture. These results provided the hormonal receptivity status of endometrium after culture and will thus serve as a reference for evaluating in vitro steroid effects on endometrium explants. Our preliminary results suggest that cultures of endometrium explants are a valid model for studying the effects of hormonal treatment on homogeneous as well as heterogeneous endometrium. These data could be particularly relevant for evaluating the potential response to hormone stimulation and treatment of endometria sampled in perimenopausal patients.
培养基中前列腺素（PG）F(2α) 的产量以及组织中的类固醇受体免疫表达。人子宫内膜样本被分为均匀和不均匀的增殖期或分泌期亚组。在增殖期子宫内膜外植体培养中，孕酮（10(-7) M）可使PGF(2α) 产量显著降低，但在分泌期子宫内膜中并非如此，而未观察到雌二醇（10(-8) M）有显著影响。培养前，通过定量免疫细胞化学评估，均匀和不均匀增殖期子宫内膜呈现出相同的雌二醇受体（ER）和孕酮受体（PR）表达模式。培养后，在外植体上检测到免疫反应性ER和PR。在未添加体外类固醇的均匀增殖期子宫内膜外植体的腺体以及不均匀增殖期子宫内膜外植体的基质中，培养后的PR免疫表达率低于培养前。在分泌期子宫内膜中，培养前后的ER或PR免疫表达率未观察到显著差异。这些结果提供了培养后子宫内膜的激素反应状态，因此将作为评估体外类固醇对子宫内膜外植体作用的参考。我们的初步结果表明，子宫内膜外植体培养是研究激素治疗对均匀和不均匀子宫内膜作用的有效模型。这些数据对于评估围绝经期患者子宫内膜对激素刺激和治疗的潜在反应可能特别相关。
