Hashiba T, Noguchi S, Tsuchiya F, Takeda M, Masuda M, Kubota Y, Hosaka M
Department of Urology, Yokohama City University School of Medicine, Yokohama, Japan.
Urol Int. 2000;64(4):209-12. doi: 10.1159/000030532.
We developed a serum-free coculture model of benign prostatic hyperplasia (BPH) to clarify whether stromal cells stimulate growth of epithelial cells from BPH tissues. Epithelial and stromal cells from freshly isolated BPH tissue were cultured separately in defined serum-free WAJC 404/RPMI 1640 medium supplemented with insulin, transferrin, selenium, hydrocortisone, bovine serum albumin, epidermal growth factor, basic fibroblast growth factor and keratinocyte growth factor. (3)H-Tdr incorporation into epithelial cells and stromal cells was used as a measure of proliferation. When epithelial cells were cocultured with stromal cells, (3)H-Tdr incorporation into epithelial cells was increased in comparison to that in epithelial cells cultured alone. Dihydrotestosterone significantly increased this effect. It is likely that the in vitro coculture model reported here will be useful for isolating and understanding stromal cell-derived paracrine growth factor(s).
我们建立了一种良性前列腺增生（BPH）的无血清共培养模型，以阐明基质细胞是否刺激BPH组织上皮细胞的生长。将新鲜分离的BPH组织中的上皮细胞和基质细胞分别培养在添加了胰岛素、转铁蛋白、硒、氢化可的松、牛血清白蛋白、表皮生长因子、碱性成纤维细胞生长因子和角质形成细胞生长因子的特定无血清WAJC 404/RPMI 1640培养基中。用（3）H-Tdr掺入上皮细胞和基质细胞作为增殖的指标。当上皮细胞与基质细胞共培养时，与单独培养的上皮细胞相比，上皮细胞中（3）H-Tdr的掺入增加。双氢睾酮显著增强了这种作用。本文报道的体外共培养模型可能有助于分离和了解基质细胞衍生的旁分泌生长因子。
