Johnson D G, Ensinck J W, Koerker D, Palmer J, Goodner C J
Endocrinology. 1975 Feb;96(2):370-4. doi: 10.1210/endo-96-2-370.
Perfusion of growth hormone inhibitory factor (somatostatin) into rat pancreas inhibited secretion of glucagon and insulin into medium containing 5.5 mM glucose. A 15-min infusion of arginine (20 mM) greatly increased glucagon and insulin secretion. When perfused simultaneously with arginine, somatostatin (55 nM) abolished the increase in glucagon secretion. The acute phase of insulin secretion in response to arginine was attenuated by somatostatin, and subsequent secretion was decreased to control levels. Pretreatment for 5 min with somatostatin blocked even acute-phase insulin secretion in response to arginine. Somatostatin did not affect basal or glucose-stimulated secretion of insulin from rat pancreatic islets isolated by the collagenase technique. Arginine-stimulated secretion of insulin was enhanced by somatostatin in isolated islets. These results demonstrate a direct effect of somatostatin on the pancreas to inhibit secretion of glucagon and insulin. The failure of somatostatin to inhibit insulin secretion in pancreatic islets may be due to alterations in the beta cells produced by the isolation procedure. It is also possible that the effect of somatostatin on insulin secretion may be mediated indirectly.
向大鼠胰腺灌注生长抑素（促生长素抑制因子）可抑制胰高血糖素和胰岛素分泌到含5.5 mM葡萄糖的培养基中。输注15分钟的精氨酸（20 mM）可大幅增加胰高血糖素和胰岛素分泌。当与精氨酸同时灌注时，生长抑素（55 nM）可消除胰高血糖素分泌的增加。生长抑素减弱了精氨酸刺激胰岛素分泌的急性期反应，随后的分泌降至对照水平。用生长抑素预处理5分钟甚至可阻断精氨酸刺激的胰岛素急性期分泌。生长抑素不影响用胶原酶技术分离的大鼠胰岛的基础胰岛素分泌或葡萄糖刺激的胰岛素分泌。在分离的胰岛中，生长抑素可增强精氨酸刺激的胰岛素分泌。这些结果表明生长抑素对胰腺有直接作用，可抑制胰高血糖素和胰岛素分泌。生长抑素未能抑制胰岛中的胰岛素分泌可能是由于分离过程对β细胞产生了改变。生长抑素对胰岛素分泌的作用也可能是间接介导的。
