Doğan B, Saarela M, Asikainen S
Institute of Dentistry, University of Helsinki, Finland.
Oral Microbiol Immunol. 1999 Dec;14(6):387-90. doi: 10.1034/j.1399-302x.1999.140611.x.
The aims of the study were to determine the genetic diversity of the rare Actinobacillus actinomycetemcomitans serotype d and to compare the ability of the repetitive extragenic palindromic element (REP)-based polymerase chain reaction (PCR) with that of the arbitrarily primed (AP)-PCR to discriminate between and within A. actinomycetemcomitans serotypes. The material included 26 A. actinomycetemcomitans serotype d isolates, 3 reference strains, and 21 A. actinomycetemcomitans isolates, representing the previously described 17 AP-PCR genotypes from 4 serotypes (a, b, c and e). Among A. actinomycetemcomitans serotype d isolates (n = 26), the AP-PCR primer distinguished 2 genotypes, whereas the REP-primer pair (REP1R-I and REP2-I) and the (GACA)4 primer each produced one genotype. Among the total of 50 A. actinomycetemcomitans isolates, REP-primer pair distinguished 6 genotypes, the primer (GACA)4 7 genotypes, and the AP-PCR 19 genotypes. Among A. actinomycetemcomitans serotype a isolates (n = 6), REP-primer pair yielded 3 genotypes and (GACA)4 and AP-PCR primer 4 genotypes, and among serotype e isolates (n = 6) 3 genotypes. All serotype b isolates (n = 7), representing the AP-PCR genotypes 2, 9, 8, 12, 13, 16 and serotype c isolates (n = 5), AP-PCR genotypes 3, 4, 14, 15, belonged to the (REP1R-I and REP2-I)-PCR genotype 4 and to the (GACA)4-PCR genotype 4. In conclusion, based on both the AP-PCR method and the less discriminative REP-PCR methods, the present genotyping results indicated limited genetic diversity among serotype d isolates of A. actinomycetemcomitans.
本研究的目的是确定罕见的伴放线放线杆菌血清型d的遗传多样性，并比较基于重复外显子回文元件（REP）的聚合酶链反应（PCR）与任意引物（AP）-PCR区分伴放线放线杆菌血清型之间及血清型内部的能力。材料包括26株伴放线放线杆菌血清型d分离株、3株参考菌株以及21株伴放线放线杆菌分离株，后者代表先前描述的来自4种血清型（a、b、c和e）的17种AP-PCR基因型。在伴放线放线杆菌血清型d分离株（n = 26）中，AP-PCR引物区分出2种基因型，而REP引物对（REP1R-I和REP2-I）以及（GACA）4引物各自产生1种基因型。在总共50株伴放线放线杆菌分离株中，REP引物对区分出6种基因型，（GACA）4引物区分出7种基因型，AP-PCR区分出19种基因型。在伴放线放线杆菌血清型a分离株（n = 6）中，REP引物对产生3种基因型，（GACA）4和AP-PCR引物产生4种基因型；在血清型e分离株（n = 6）中产生3种基因型。所有血清型b分离株（n = 7），代表AP-PCR基因型2、9、8、12、13、16，以及血清型c分离株（n = 5），AP-PCR基因型3、4、14、15，均属于（REP1R-I和REP2-I）-PCR基因型4和（GACA）4-PCR基因型4。总之，基于AP-PCR方法以及区分能力较弱的REP-PCR方法，目前的基因分型结果表明伴放线放线杆菌血清型d分离株的遗传多样性有限。
