Erdogan S, Ozer A Y, Ercan M T, Hincal A A
Faculty of Pharmacy, Department of Radiopharmacy, Hacettepe University, Ankara, Turkey.
J Microencapsul. 2000 Jul-Aug;17(4):459-65. doi: 10.1080/026520400405714.
In this study, liposomes containing glutathione were evaluated to detect infection in mice. Glutathione liposomes were labelled by using 99mTc-labelled-HMPAO (hexamethyl propylamine oxime). Mice were infected in the thigh by intramuscular injection with turbentine. Labelled liposomes were applied to the tail vein of the mice intravenously. At fixed time intervals they were sacrificed. The animals were imaged under a gamma camera. Then, tissue samples were excised and radioactivity of all organs was counted. Abscess-to-muscle, abscess-to-liver, and abscess-to-spleen ratios were calculated. The ratios of abscess-to-muscle were found to be 1.6 and 11.6 at 1 h and 24 h, respectively. According to these data, the abscess can be defined at 1 h and it became more clear with time.
在本研究中，对含有谷胱甘肽的脂质体进行评估，以检测小鼠体内的感染情况。谷胱甘肽脂质体通过使用99mTc标记的HMPAO（六甲基丙二胺肟）进行标记。通过在小鼠大腿处肌肉注射松节油使其感染。将标记的脂质体静脉注射到小鼠的尾静脉中。在固定的时间间隔处死小鼠。在γ相机下对动物进行成像。然后，切除组织样本并对所有器官的放射性进行计数。计算脓肿与肌肉、脓肿与肝脏以及脓肿与脾脏的比值。脓肿与肌肉的比值在1小时和24小时时分别为1.6和11.6。根据这些数据，在1小时时即可确定脓肿，并且随着时间推移会变得更加清晰。
