Jośko J, Hendryk S, Jedrzejowska-Szypułka H, Słowiński J, Gwóźdź B, Lange D, Harabin-Słowińska M
Department of Physiology Silesian University School of Medicine, Zabrze, Poland.
J Physiol Pharmacol. 2000 Jun;51(2):241-9.
Aim of the study was to quantify cerebral vasospasm in rats after subarachnoid hemorrhage (SAH) by morphometric examination of basilar artery and to evaluate the influence of endothelin receptor blocker BQ-123 on basilar artery constriction. The rat cisterna magna (CM) was cannulated and after 7 days SAH was developed by administration of 100 microl autologic, non-heparinized blood to the CM. The sham subarachnoid hemorrhage was developed by intracisternal administration of 100 microl of artificial cerebrospinal fluid. Endothelin receptor blocker BQ-123 was injected into the CM in a dose of 40 nmol diluted in 50 microl of cerebrospinal fluid 20 min. before SAH, and 24h and 48 h after SAH. After perfusion fixation the brains were removed from the skull and histological preparations of basilar artery were done. The internal diameter and wall thickness of basilar arteries was measured by interactive morphometric method. The most severe vasospasm was found in rats after SAH. The presence of numerous infiltrations composed of neutrophils and macrophages correlated with advanced vasospasm (index of constriction 5 times lower than in normal), suggesting the role of other factors participating in the late phase of vasospasms after SAH. Administration of BQ-123 in the late phase after SAH caused the dilatation of basilar artery. Following the administration of BQ-123 in the late phase (48 h after SAH) the basilar artery dilated, its wall became thinner, and the number of leukocyte infiltrations in the subarachnoid space decreased compared to the values after SAH alone.
本研究的目的是通过对基底动脉进行形态计量学检查来量化大鼠蛛网膜下腔出血（SAH）后的脑血管痉挛，并评估内皮素受体阻滞剂BQ-123对基底动脉收缩的影响。将大鼠枕大池（CM）插管，7天后通过向CM内注入100微升自体、非肝素化血液诱导SAH。通过向脑池内注入100微升人工脑脊液诱导假蛛网膜下腔出血。在SAH前20分钟、SAH后24小时和48小时，将内皮素受体阻滞剂BQ-123以40纳摩尔的剂量稀释于50微升脑脊液中注入CM。灌注固定后，将大脑从颅骨中取出并制作基底动脉的组织学标本。通过交互式形态计量学方法测量基底动脉的内径和壁厚。在SAH后的大鼠中发现了最严重的血管痉挛。由中性粒细胞和巨噬细胞组成的大量浸润的存在与严重的血管痉挛相关（收缩指数比正常情况低5倍），提示其他因素在SAH后血管痉挛后期发挥作用。在SAH后期给予BQ-123可导致基底动脉扩张。在SAH后期（SAH后48小时）给予BQ-123后，基底动脉扩张，其壁变薄，蛛网膜下腔白细胞浸润数量比单纯SAH后减少。
