Seppo L, Karjala K, Nevala R, Korpela R, Lähteenmäki T, Solatunturi E, Tikkanen M J, Vapaatalo H
Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki, Finland.
J Physiol Pharmacol. 2000 Jun;51(2):251-65.
Both LDL oxidation and LDL fatty acid composition affect vascular relaxation and contraction. The aim of this study was to investigate whether long-lasting dietary habits (vegetarian, fish and high saturated fat as a control group) can change those properties of partially oxidized LDL (ox-LDL) which are reflected in altered vascular responses measured with a bioassay. The effects of ox-LDL were investigated on rat mesenteric arteries. In endothelium intact arterial rings the contractile responses to noradrenaline (NA) tended to be diminished in the presence of ox-LDL derived from the fish diet group compared with the other groups. In the endothelium denuded arterial rings the contractile responses to NA and KCl were significantly enhanced by ox-LDL from the fish diet group compared with the control group. The ox-LDL from the fish diet group increased the diclofenac, L-NAME resistant relaxations to ACh compared to the control diet group suggesting the role of endothelium derived hyperpolarizing factor (EDHF). In conclusion, partially oxidized LDL from subjects living on a fish diet is biologically more vasoactive in bioassay systems than partially oxidized LDL from those living on vegetarian or saturated fatty acid containing diets. The impaired responses in vasoconstriction and improved vasodilation seem to be endothelium dependent.
低密度脂蛋白（LDL）氧化和LDL脂肪酸组成均会影响血管舒张和收缩。本研究旨在调查长期饮食习惯（素食、鱼类饮食以及以高饱和脂肪饮食作为对照组）是否会改变部分氧化LDL（ox-LDL）的这些特性，这些特性可通过生物测定法检测到的血管反应改变体现出来。研究了ox-LDL对大鼠肠系膜动脉的影响。在内皮完整的动脉环中，与其他组相比，来自鱼类饮食组的ox-LDL存在时，对去甲肾上腺素（NA）的收缩反应往往会减弱。在内皮剥脱的动脉环中，与对照组相比，来自鱼类饮食组的ox-LDL显著增强了对NA和氯化钾（KCl）的收缩反应。与对照饮食组相比，来自鱼类饮食组的ox-LDL增加了双氯芬酸、对乙酰胆碱（ACh）的L- NAME抗性舒张，提示内皮衍生超极化因子（EDHF）的作用。总之，在生物测定系统中，以鱼类饮食为主的受试者的部分氧化LDL比以素食或含饱和脂肪酸饮食为主的受试者的部分氧化LDL具有更强的生物血管活性。血管收缩反应受损和血管舒张改善似乎依赖于内皮。
