三磷酸腺苷：猪肝烟酰胺单核苷酸腺苷酰转移酶。纯化及性质
Adenosine triphosphate: nicotinamide mononucleotide adenylyltransferase of pig liver. Purification and properties.
作者信息
Ferro A M, Kuehl L
出版信息
Biochim Biophys Acta. 1975 Dec 18;410(2):285-9. doi: 10.1016/0005-2744(75)90231-4.
PMID:1090
Abstract
Adenosine triphosphate : nicotinamide mononucleotide adenylyltransferase (EC 2.7.7.1) has been purifiec approximately 3500-fold from an extract of pig liver nuclei to a specific activity of 40 mumol of NAD+ per min per mg protein. The enzyme was found to have a molecular weight of 203 000, a frictional ratio of 1.6 and an isoelectric point of approximately 5. Michaelis constants for ATP and NMN were 0.11 mM and 0.12 mM, respectively.
摘要
三磷酸腺苷
烟酰胺单核苷酸腺苷酰转移酶（EC 2.7.7.1）已从猪肝细胞核提取物中纯化了约3500倍，比活性达到每毫克蛋白质每分钟40微摩尔NAD⁺。发现该酶的分子量为203000，摩擦比为1.6，等电点约为5。ATP和NMN的米氏常数分别为0.11 mM和0.12 mM。
Zotero 插件