Godoy F, Zenteno P, Cerda F, Gonzalez B, Martinez M
Laboratorio de Microbiologia, Facultad de Ciencias Biologicas, Universidad de Concepcion, Chile.
Chemosphere. 1999 Feb;38(3):655-62. doi: 10.1016/s0045-6535(98)00205-7.
The effect of 2,4,5- and 2,4,6-trichlorophenol on the microbiota from a polluted and a pristine site of a river was studied. Bacterial metabolic activity measurements by epifluorescence microscopy showed that the polluted site contained more metabolically active cells than the pristine site. Total culturable bacterial counts and tolerant bacterial counts from both sites were not affected by incubation (for up to 5 days) with 200 ppm of chlorophenols. However, the incubation with 500 ppm of 2,4,5-trichlorophenol prevented detection of total and tolerant bacterial counts in the pristine site, and inhibited tolerants in the polluted site. None of 250 bacterial colonies directly isolated from these samples was able to grow on chlorophenols. However, bacteria able to grow on 2,4,6-trichlorophenol, were obtained by enrichment of water and sediments samples.
研究了2,4,5-三氯苯酚和2,4,6-三氯苯酚对一条河流污染位点和原始位点微生物群的影响。通过落射荧光显微镜测量细菌代谢活性表明，污染位点的代谢活性细胞比原始位点更多。两个位点的可培养细菌总数和耐受细菌数不受200 ppm氯酚孵育（长达5天）的影响。然而，500 ppm的2,4,5-三氯苯酚孵育阻止了原始位点可培养细菌总数和耐受细菌数的检测，并抑制了污染位点的耐受细菌。直接从这些样品中分离出的250个细菌菌落均不能在氯酚上生长。然而，通过富集水样和沉积物样品，获得了能够在2,4,6-三氯苯酚上生长的细菌。
