植物钾离子通道在哺乳动物细胞中的温度依赖性功能表达
Temperature-dependent functional expression of a plant K(+) channel in mammalian cells.
作者信息
Szabò I, Negro A, Downey P M, Zoratti M, Lo Schiavo F, Giacometti G M
机构信息
Department of Biology, CRIBI, CNR Unit for Biomembranes, University of Padua, Via G. Colombo 3, Padua, 35121, Italy.
出版信息
Biochem Biophys Res Commun. 2000 Jul 21;274(1):130-5. doi: 10.1006/bbrc.2000.3095.
The Arabidopsis thaliana potassium channel KAT1 was expressed and characterized in Chinese hamster ovary cells. KAT1-GFP fusion protein was successfully targeted to the plasma membrane and electrophysiological analysis revealed functional expression of KAT1 only in cells cultured at 30 degrees C. The main biophysical characteristics of KAT1 are similar to those described for the channel expressed in other systems. CHO cells represent an advantageous expression system and may be the system of choice to study the expression, assembly, function, and regulation of plant potassium channels in general.
拟南芥钾通道KAT1在中国仓鼠卵巢细胞中进行了表达和特性分析。KAT1-GFP融合蛋白成功靶向到质膜，电生理分析表明KAT1仅在30摄氏度培养的细胞中功能性表达。KAT1的主要生物物理特性与在其他系统中表达的该通道所描述的特性相似。中国仓鼠卵巢细胞代表了一种有利的表达系统，总体而言可能是研究植物钾通道的表达、组装、功能和调节的首选系统。
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