在流动相中使用核酸，通过高效液相色谱-电化学检测法测定食品中的抗坏血酸。
Use of nucleic acids in the mobile phase for the determination of ascorbic acid in foods by high-performance liquid chromatography with electrochemical detection.
作者信息
Iwase H
机构信息
Ajinomoto Co., Inc., Central Research Laboratories, Kawasaki, Japan.
出版信息
J Chromatogr A. 2000 Jun 9;881(1-2):327-30. doi: 10.1016/s0021-9673(00)00057-1.
The sodium salts of amino acids, nucleic acids and organic acids were examined in a new mobile phase for the determination of ascorbic acid (AA) in foods. It was possible to use disodium guanosine-5'-monophosphate (GMP) (20 mM GMP, pH 2.1) in a new mobile phase after comparison of five mobile phases. The proposed method is simple, rapid (analysis time: ca. 6 min), sensitive (detection limit: ca. 0.1 ng per injection (5 microl) at a signal-to-noise ratio of 3), highly selective and reproducible [relative standard deviation: ca. 2.7% (n=7)]. The calibration graph of AA was linear in the range of 0.1 to 50 ng per injection (5 microl). Recovery of AA was over 90% by the standard addition method.
在一种用于测定食品中抗坏血酸（AA）的新型流动相中，对氨基酸、核酸和有机酸的钠盐进行了检测。在比较了五种流动相之后，发现可以在新型流动相中使用5'-鸟苷酸二钠（GMP）（20 mM GMP，pH 2.1）。所提出的方法简单、快速（分析时间：约6分钟）、灵敏（检测限：在信噪比为3时，每次进样（5微升）约0.1纳克）、具有高度选择性且可重现[相对标准偏差：约2.7%（n = 7）]。AA的校准曲线在每次进样（5微升）0.1至50纳克的范围内呈线性。通过标准加入法，AA的回收率超过90%。
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