分离代谢物转运或利用缺陷型突变体的方法。
Procedure for isolating mutants defective in metabolite transport or utilization.
作者信息
Berg C M, Rossi J J, Coleman J V, Conlon M
出版信息
J Bacteriol. 1975 Mar;121(3):1216-8. doi: 10.1128/jb.121.3.1216-1218.1975.
Abstract
Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in the presence of a low level of the required metabolite.
摘要
在低水平所需代谢物存在的非许可条件下，通过青霉素富集，可轻松从（针对该代谢物的）兼性营养缺陷型菌株中获得在摄取或利用特定代谢物方面存在缺陷的突变体。
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本文引用的文献
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2
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Biochim Biophys Acta. 1959 Apr;32:582-3. doi: 10.1016/0006-3002(59)90650-x.
3
OSMOTIC-REMEDIAL MUTANTS. A NEW CLASSIFICATION FOR NUTRITIONAL MUTANTS IN YEAST.渗透补救突变体。酵母营养突变体的一种新分类。
Genetics. 1964 Nov;50(5):829-39. doi: 10.1093/genetics/50.5.829.
4
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Biochim Biophys Acta. 1960 Aug 26;42:535-8. doi: 10.1016/0006-3002(60)90836-2.
5
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J Biol Chem. 1956 Jan;218(1):97-106.
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7
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