Koga D, Mitsutomi M, Kono M, Matsumiya M
Department of Biological Science, Faculty of Agriculture, Yamaguchi University, Japan.
EXS. 1999;87:111-23. doi: 10.1007/978-3-0348-8757-1_8.
Chitinases are found in many organisms, and their properties seem to be closely related to their biological function. In this chapter, the physicochemical properties of chitinases such as molecular size are compared among organisms, and the optimum and stability conditions for chitinase activity are described. Furthermore, considering their classification based on amino acid sequence, kinetic behaviors are discussed together with their biological functions. In particular, hydrolytic mechanisms such as inversion and retention of the substrate are discussed in relation to allosamidin inhibition.
几丁质酶存在于许多生物体中,其特性似乎与其生物学功能密切相关。在本章中,将比较不同生物体中几丁质酶的物理化学性质,如分子大小,并描述几丁质酶活性的最佳条件和稳定性条件。此外,基于氨基酸序列对其进行分类,讨论了动力学行为及其生物学功能。特别是,结合别洛沙米定抑制作用,讨论了底物的转化和保留等水解机制。